TABLE 1.
Effect of glucose or snf1Δ on meiosis
| Genotypea | Mediumb | Frequency of intragenic recombinants (10−5)c | % of cells forming sporesd |
|---|---|---|---|
| Wild type | Growth | 0.6 ± 0.2 | 0 |
| OAc | 60 ± 11 | 47 ± 2 | |
| OAc + Glu | 0.4 ± 0.1 | 0 | |
| snf1Δ | OAc | 0.4 ± 0.1 | 0 |
| OAc + Glu | 0.6 ± 0.2 | 0 | |
| Wild type(YEp-IME1) | OAc | 67 ± 2 | 48 ± 3 |
| Glu | 17 ± 3 | 6 ± 3 | |
| snf1Δ(YEp-IME1) | OAc | 4.2 ± 0.9 | 0 |
| Glu | 7.8 ± 0.8 | 0.2 ± 0.1 |
The wild type is SH777, snf1Δ is snf1Δ::URA3/snf1Δ::URA3, and wild type (YEp-IME1) and snf1Δ(YEp-IME1) contain the YEp351-IME1 plasmid. Strains are otherwise isogenic.
Growth, YPA medium; OAc, sporulation medium; OAc + Glu, sporulation medium with 0.4% glucose added; Glu, 2% glucose.
Commitment to intragenic recombination between trp1 heteroalleles (Trp+ prototrophs/CFU) after 36 h in growth medium or after an additional 24 h in OAc, OAc + Glu, or Glu. Percentages of cell viability for each of the nine cultures were as follows (from top to bottom): 38 ± 2, 37 ± 1, 49 ± 1, 33 ± 1, 38 ± 3, 34 ± 5, 30 ± 6, 33 ± 1, and 19 ± 1.
Percentages of the total population of cells that formed asci, measured at the same times as recombination.