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. 2023 Dec 21;134(5):e174508. doi: 10.1172/JCI174508

Figure 6. TMEM63A/B are essential for mechanosensitive currents in AECs.

Figure 6

(A) SACs were abolished in AT1 cells, AT2 cells, and LB/EL from Tmem63a/b-cDKO mice. Current amplitudes at –80 mV and –80 mmHg for AT1 and AT2 cells and –60 mV and –60 mmHg for LB/EL were used for comparison. n = 13 (Ctrl-AT1, from Ctrl-63ab mice); n = 18 (KO-AT1, from Ager-63ab); n = 25 (Ctrl-AT2, from Ctrl-63ab); n = 21 (KO-AT2, from Sftpc-63ab); n = 16 (Ctrl-LB/EL, from Ctrl-63ab); n = 13 (KO-LB/EL, from Sftpc-63ab) cells. **P < 0.01 and ***P < 0.001, by 2-tailed, unpaired Student’s t test. (B) Immunofluorescence images of endogenous TMEM63B with N-terminal 2×V5-tag–knockin (N-V5-KI) in an AT2 cell in a frozen section of mouse lung. LAMP1 is a marker of LBs. Scale bar: 5 μm. (C) Amplitudes of SACs in control and human TMEM63B-transfected (h63B-transfected) HeLa cells. Holding potential: –80 mV; pressure: –80 mmHg. n = 18 and 20 for HeLa and HeLa-h63B cells, respectively. **P < 0.01, by 2-tailed, unpaired Student’s t test. (D) Representative traces of SACs under the cell-attached configuration in a h63B-transfected HeLa cell. The pressure-current relationship is shown in the inset. (E and F) SAC traces and current-voltage relationships of inside-out recordings from h63B-transfected HeLa cells suggesting selectivity for Na+ and K+ over Ca2+.