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. Author manuscript; available in PMC: 2024 Dec 20.
Published in final edited form as: J Am Chem Soc. 2023 Dec 6;145(50):27521–27530. doi: 10.1021/jacs.3c09039

Figure 3.

Figure 3.

Concentration correlations of different labeling schemes. Correlations between the 2H−/(13C− + 12C−) and 13C−/(13C− + 12C−) ratios for each lipid mixture. The 2H−/(13C− + 12C−) ratio tracks the amount of 2H-labeled lipid in the bilayer, while the 13C−/(13C− + 12C−) ratio tracks the amount of 13C-labeled lipid in the bilayer. (A) Correlation between 2H31-POPC and 13C18-DSPC ratios in the ternary mixture DSPC:13C18-DSPC:POPC:2H31-POPC:CHOL 20:20:20:20:20. (B) Correlation between 2H31-POPC and 13C18-POPC ratios in the ternary mixture DSPC:13C18-POPC:2H31-POPC:CHOL 40:20:20:20. (C) Absence of correlation between 2H31-POPC and 13C18-POPC ratios in a pure POPC mixture with composition 13C18-POPC:2H31-POPC:POPC 20:20:60.