Figure 2.

Plaque reduction assay for evaluating inactivation of SARS-CoV-2 virus in virus-infected cells using different chemical agents

The SARS-CoV-2 WA1/2020 strain from virus-infected cells was inactivated using (A) RIPA buffer (NP-40), (B) PFA, and (C) virus-infected ALO’s were inactivated using Parse reagent, and untreated controls followed by titration on VeroE6 or VeroE6-TMPRSS2 cell lines at different dilution. Cells were fixed and stained at 72 h post-infection with crystal violet. The experiment for each inactivation was repeated twice, and representative images are shown.