Figure 4.

Schematic of plaque assays and crystal violet staining

The procedure includes seeding of cells in a 6-, 12-, or 24-well plate for the experiment. The inactivated virus and their respective controls are serially diluted (wherever mentioned) and inoculated to cells and incubate to facilitate viral attachment (virus adsorption), add an avicel or CMC overlay. Incubate the plate in the incubator for 72 h at 37°C, to allow plaque formation; Fix infected cells with ethanol and stain the cells with crystal violet.