Table 1.
Chemical Reagents/Conditions | Final concentration | Incubation time | Temperature | Reference |
---|---|---|---|---|
Heat | N/A | 30 min | 65°C | Batéjat et al.3 |
Triton X-100 | 1% | 20 min | 20°C–25°C | Case et al.4 |
Isopropanol | 30% | 30 min | 20°C–25°C | Kratzel et al.,5 Meyers et al.6 |
Methanol | 50% | 10 min | 20°C–25°C | Kariwa et al.,7 Welch et al.,8 Patterson et al.9 |
MTM | 1:3 v/v | 10 min | 20°C–25°C | Welch et al.8 |
RIPA (NP-40) | 0.5% | 30 min | 20°C–25°C | Welch et al.8 |
PFA | 4% | 20 min | 20°C–25°C | Möller et al.,10 Eddins et al.11 |
Parse reagent | 100% | 10 min | 20°C–25°C | Flamier et al.12 |
n/a = Not applicable.