Table 2.

Calculation of viral titer and the effectiveness of different chemical reagents in inactivating SARS-CoV-2 virus

Chemical Reagents/Conditions	Virus only/+PBS condition			Number of plaques in virus + inactivating agents	Log10 reduction
	Number of plaques	Dilutions (10-fold)	Titers (PFU/mL)
Heat	N/A	N/A	N/A	n/d	N/A
Triton X-100	1	5	6.66 × 105	n/d	>5 logs
Isopropanol	4	4	2.66 × 105	n/d	>5 logs
Methanol	19	4	1.27 × 106	n/d	>6 logs
MTM	6	3	4.00 × 104	n/d	>4 logs
RIPA (NP-40)	3	4	2.00 × 105	n/d	>5 logs
PFA	N/A	N/A	N/A	n/d	N/A
Parse reagent	2	3	1.33 × 104	n/d	>4 logs

n/a = Not applicable: entire volume of sample was used for plaque assay that led to the complete elimination of viral population and no dilution series was carried out.

n/d = Not detected, due to complete inactivation of viruses.