2X Minimum Essential Medium (MEM) medium
| Reagent | Final concentration | Amount |
|---|---|---|
| MEM | N/A | 500 mL |
| Bovine Serum Albumin (BSA) | 3% | 6 mL |
| L-glutamate | 2% each | 10 mL each |
| Pen-Strep | ||
| HEPES | ||
| Sodium Bicarbonate (NaHCO3) | 5% | 16 mL |
| Total | N/A | 552 mL |
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