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Cyclin A2 and E1 complementation rescues C70 impairment of 3T3-L1 preadipocyte differentiation. 3T3-L1 preadipocytes were transfected at day −3 with expression plasmids for Ccna2 or Ccne1 and treated with C70 or Veh on days −2 to 0. (A) Lipid staining with oil red O (ORO) and quantification in 3T3-L1 at day 8 of differentiation. (B) Dlk1 mRNA levels at day 2. (C) Adipogenic gene expression at day 8. Analysis by ANOVA followed by pairwise t test; * P < .05; ** P < .01; *** P < .001.
