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. 2024 Jan 30;300(3):105695. doi: 10.1016/j.jbc.2024.105695

Figure 5.

Figure 5

Expression of PPM1s in BHLHE40-modulated EC cells.A–D, immunoblotting analysis of EC cells cultured for 24 h in DMEM or DMEM:F12 with the indicated concentrations of glucose with 1 mM sodium pyruvate and 10% FBS. HHUA (A), KLE (B). HEC-1 (C), and Ishikawa (D) cells. A–D, values under panels indicate relative expression levels of PPM1A/ACTB, PPM1F/ACTB, and PPM1E/ACTB. A–D, data are representative of three biological replicates. E–L, real-time RT-PCR analysis of EC cells cultured for 24 h in DMEM or DMEM:F12 with 1.0 g/L glucose, 1 mM sodium pyruvate, and 10% FBS. HHUA (E and F), KLE (G and H). HEC-1 (I and J), and Ishikawa (K and L) cells. PPM1A (E, G, I, and K), PPM1F (F, H, and J), and PPM1E (L). E–L, data are from three biological replicates. Unpaired two-sided Student’s t test was used. ∗p < 0.05; ∗∗p < 0.01; ∗∗∗p < 0.001. LtCtrl, LtControl; LtE40, LtBHLHE40; shCtrl, shControl; shE40, shBHLHE40.