Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2024 Jan 30;300(3):105695. doi: 10.1016/j.jbc.2024.105695

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2024 The Authors

This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).

PMC Copyright notice

Phosphatase activity of PPM1F on phospho-AMPKα Ser172.A, immunoblotting of HHUA cells transfected with FLAG-tagged PPM1A-, PPM1B-, PPM1E-, or PPM1F-expressing vector. B, immunoblotting of HHUA cells transfected with wild type and phosphatase inactive mutant (R326A-I328A) PPM1F. C, in vitro phosphatase assay reconstituted with FLAG-PPM1F and activated AMPK complex. D and E, immunoblotting analysis of HHUA (D) and HEC-1 (E) cells transfected with indicated siRNAs. Values under panels indicate relative expression levels of p-AMPKα/AMPKα, p-ACC/ACC, p-PDHA1/PDHA1, p-LDHA/LDHA, LDHA/ACTB, BHLHE40/ACTB, PPM1A/ACTB, and PPM1F/ACTB. Data are representative of at least two technical replicates from three biological replicates.