Figure 4.

Kinetics of transgene expression by rSFV-infected cells in cancer-spheroids

(A) The setup of the cancer-spheroid generation and infection assay to measure transgene (GFP) expression using microscopy. Representative images of cancer-spheroids generated from LNCaP (B) and PANC-1 cells (C), and microscopy-based temporal quantification of GFP+ cells in cancer-spheroids from LNCaP and PANC-1 cells (on the right).

(D) Confocal microscopy-based characterization of spatial information of GFP+ cells present in an LNCaP spheroid (on the left). The depth at which a GFP-expressing cell is present in the spheroid is depicted by the color-coded legend (on the right). In (B and C) the top row depicts brightfield images, the bottom row depicts fluorescence images for GFP visualization, and the plots represent data from 8 replicate-spheroids. See Figure S4 for microscopy images corresponding to (B and C). Data are presented as mean ± SEM.