FIG. 2.

SCp2–Id-1 cells invade the ECM and migrate in a Boyden chamber. Parental SCp2 cells (lane 1), SCp2 cells transfected with an insertless vector (lane 2), SCp2–Id-1 cells (lane 3), and SCp2 cells transfected with Id-1 in the antisense orientation (lane 4) were plated on ECM-coated filters in Boyden chambers; the number of cells that migrated through the filter after 16 to 20 h was determined as described in Materials and Methods. Error bars indicate standard deviations from three or four independent fields. The data shown are from one of five independent experiments which showed very similar differences among the cell types.