Table 2.
Different parameters for HPTLC method validation.
| S. no | Parameters | Piperine |
|---|---|---|
| 1 | Linearity range (µg /spot; n = 12a) | 2–10 |
| 2 | Correlation coefficient (r2) | 0.9957 |
| 3 | Regression equation | Y = 15.476*X + 7911 |
| 4 | Calculated SD value (CATS software) | 2.82 |
| 5 | b Limit of detection (LOD) (ng) [3 × SD/S] | 140 |
| 6 | b Limit of quantification (LOQ) (ng) [10 × SD/S] | 1200 |
| 7 | Rf and λmax (nm) | 0.63 and 340 |
| Precision and accuracy | ||
| 8 | Intra-day RSD (%), n = 5 | 0.23 |
| 9 | Inter-day RSD (%), n = 5 (day-1/day-2/day-3) | 0.48 |
| Recovery | ||
| 10 | Amount of standard in fruit samples (µg mg−1) containing highest Piperine | 13.302 |
| 11 | Amount of standard added in fruit sample (µg mg−1) | 17, 34, 51 |
| 12 | Amount of standard found (µg) | 50.58, 67.62, 84.64 |
| 13 | Recovery (%) | 99.98, 100.04, 100.05 |
aFour concentration levels in triplicates.
bSD is the standard deviation of the blank response and S is the slope of the calibration curve.