FIG. 3.

(A) The IL-9-responsive cell lines indicated were cultured in the presence of saturating concentrations of the indicated cytokines for at least 3 days. After electrophoresis of 10 μg of total RNA and transfer to nitrocellulose, the filter was hybridized with a specific ³²P-labelled mouse adseverin cDNA probe. Hybridization with a β-actin probe was used as a control to compare the amounts of RNA in the lanes. (B) Selective amplification of adseverin (D5) cDNA. RT-PCR of total RNA of IL-9-induced TS2 cells. pDpcr was used as the forward primer. The sequences of the different primers are described in Materials and Methods. Lanes: 1, primer D3; 2, primer D5; 3, primer D7; 4, primer D10; 5, primer D15; 6, primer D20; 7, primer d5/25; 8, primer d10/20; 9, primer Dcontr1; 10, primer Dcontr2.