TABLE 2.
Sample | Falling time(s) |
---|---|
Negative control | ∞ (>60 min) |
Mouse adseverin | 4 |
Mouse adseverin (D5) | 12 |
Gelsolin | 5 |
The total volume was 200 μl. The actin concentration was 1.2 mg/ml. The molar ratio of actin to actin binding protein was 1:500. Actin was polymerized in a capillary in the presence or absence of actin binding protein. After 30 min, the time necessary for a ball to move 3 cm was measured.