Figure 2.

Fluorescence-melting curves and corresponding T_ms of the MBs FMB1–5 in the absence of a DNA target. The concentrations of MBs were 200 nM in 50 mM KCl, 10 mM Tris and 3.5 mM MgCl₂, pH 8.0. The monophasic transitions reflect hairpin to random coil transitions.