Table 2.
MBs and reference sequences prepared and investigated as well as their ESI− mass-spectrometric characterization
| Molecular beacons | (M − H)− calculated | (M − H)− found | |
|---|---|---|---|
| FMB1 | FAM-GCGCGAAGTTAAGACCTATGCGCGC-Dabsyl | 8726.9 | 8727.2 |
| FMB2 | FAM-CGCGAAGTTAAGACCTATGCGCG-Dabsyl | 8107.6 | 8108.5 |
| FMB3 | FAM-TCGCGAAGTTAAGACCTATGCGCGA-Dabsyl | 8725.0 | 8725.4 |
| FMB4 | FAM-aaaaAAGTTAAGACCTATGaaaa-Dabsyl | 8252.6 | 8254.2 |
| FMB5 | FAM-aaaaaAAGTTAAGACCTATGaaaaa-Dabsyl | 8906.4 | 8906.8 |
| Cy5MB1 | Cy5-NH2C3-CCGGTGGGTTGCTTTGAAGCCGG-Dabcyl | 8364.0 | 8362.7 |
| Cy5MB2 | Cy5-NH2C3-CCGGTGGGTTGCATTGAAGCCGG-Dabcyl | 8373.0 | 8373.3 |
| Cy5MB3 | Cy5-NH2C3-CCGCTGGGTTGCATTGAAGGCGG-Dabsyl | 8409.0 | 8409.7 |
| Cy5MB4 | Cy5-NH2C3-GCGGTGGGTTGCATTGAAGCCGC-Dabcyl | 8373.0 | 8373.8 |
| Cy5MB5 | Cy5-NH2C3-GCGCTGGGTTGCATTGAAGGCGC-Dabcyl | 8373.0 | 8373.5 |
| Cy5MB6 | Cy5-NH2C3-aaaaTGGGTTGCTTTGAAGaaaa-Dabsyl | 8544.4 | 8544.9 |
| Cy5MB7 | Cy5-NH2C3-aaaaTGGGTTGCATTGAAGaaaa-Dabsyl | 8553.4 | 8554.0 |
| Cy5Ref | Cy5-NH2C3-CTTTTGAGCGTGAGC | 5375.0 | 5373.9 |
Upper case letters, 2′-deoxynucleotides; lower case letters, homo-DNA nucleotides; bold, probe region; italics, stem region; bold underline, mismatched base.