Fig. 5. Following biosensing on a microscope.

A Confocal fluorescence microscopy image of R₉-ATP coacervates with HP form MB sequestered into the coacervates. Excitation 520 nm, detection 540–740 nm. B Fluorescence spectra obtained from confocal fluorescence microscopy of coacervates seen in A at different times upon addition of a 1:2 ratio of MB:target. Spectra are obtained by averaging over the entire field of view. C Time trace of Cy5/Cy3 fluorescence ratio of confocal image seen in A.