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Fluorescence quantum yield of the dyes on the MB in varying confirmations (HP and dsDNA) and conditions (buffer and coacervate)
| Buffer | Coacervate | |||
|---|---|---|---|---|
| HP | dsDNA | HP | dsDNA | |
| Cy3 donor | 0.199 ± 0.01 | 0.16 ± 0.01 | 0.52 ± 0.04 | 0.62 ± 0.04 |
| Cy5 acceptor | 0.20 ± 0.01 | 0.28 ± 0.01 | 0.39 ± 0.03 | 0.36 ± 0.02 |
Determinations based on secondary standards (rhodamine B and oxazine 720)30, uncertainties are based on propagating uncertainties from standards and experimental spectroscopy.
