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. 2024 Feb 29;14:4967. doi: 10.1038/s41598-024-55315-y

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© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Effect of local delivery of 7 nM AaH-II on the somatic and AIS AP in L5 pyramidal neurons. (A) On the top, somatic AP elicited by current pulse injection in a L5 pyramidal neuron in control condition (black trace) and after local delivery of 7 nM AaH-II (grey trace). On the bottom, AIS (~ 10–30 µm from the soma) AP in the same cell in control condition (red trace) and after AaH-II delivery (purple trace). Wid parameters used for the analysis are indicated for the soma and AIS relative to the control traces. (B) Scatter plots and mean ± SD (N = 9 cells) of the soma (black lines and symbols) and AIS (red lines and symbols) Wid changes following AaH-II addition. “*” indicates that the change was significantly larger in the AIS (p = 3.4·10^–4, paired t-test).