Table 2.
Flow cytometry panels.
| Panel | Antigens tested | Fluorochromes |
|---|---|---|
| T-cell |
CD2, CD3, CD4, CD5, CD7, CD8, CD19, CD45, TCRγδ, TRBC1, TRBC2 |
PerCP-Cy5.5, PE-Cy7, AF-700, BV-605, BV-421, APC-H7, BV-711, V500, PE, FITC, AF-647 |
| Sezary |
CD2, CD3, CD4, CD5, CD7, CD8, CD19, CD45, CD26, TRBC1, TRBC2 |
PerCP-Cy5.5, PE-Cy7, AF-700, BV-605, BV-421, APC-H7, BV-711, V500, PE, FITC, AF-647 |
| Vβ/TRBC | Vβx, Vβy, CD3, TRBC1, TRBC2 (x 8 tubes) | FITC, PE, PE-Cy7, BV-605, AF-647 |
|
Naïve/ memory |
CD3, CD4, CD8, CD45RA, CD62L, TRBC1, TRBC2 | BV-605, AF-700, APC-H7, PE-Cy7, PE, FITC, AF-647 |
Antigens and fluorochromes are listed in corresponding order.