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. 2024 Feb 9;27(3):109166. doi: 10.1016/j.isci.2024.109166

Figure 3.

Figure 3

Quantification of TDP-43 levels in total, soluble, and insoluble protein fractions

(A) Schematics representing the fractionation workflow into total (unfractionated), soluble (RIPA), and insoluble (urea) protein fractions.

(B–F) Immunoblot (B) and quantification of TDP-43 (C and D) and C-terminal fragment of 35 kDa (CTF-35) (E and F) levels in soluble and insoluble fractions. Vinculin (soluble) and βIII-tubulin were used as fractionation and loading controls, respectively.

(G–J) Immunoblot (G and I) and quantification of total levels of TDP-43 (H) and phosphorylated TDP-43 (Ser409/410) (J) in unfractionated lysates. Actin was used as loading control. All data shown as mean ± SEM. Extractions were performed in MNs harvested after 6 weeks post-plating. n = 4 independent experiments. Ordinary one-way ANOVA. See also Figure S5.