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. 2024 Feb 9;27(3):109166. doi: 10.1016/j.isci.2024.109166

Figure 5.

Figure 5

TDP-43 MNs show progressive alterations in spontaneous neuronal activity

(A) Representative phase-contrast images of MNs differentiated for 6 weeks on 24-well MEA plates. Scale bar, 250 μm.

(B) Longitudinal changes in mean firing rate of MNs recorded weekly over a span of 8 weeks post-plating. n = 11 independent experiments.

(C) Spontaneous neuronal activity of MN cultures differentiated for 6 weeks recorded for 300 s shown as raster plot and spike histogram. Individual spikes are shown in black and bursts are shown in blue.

(D) Effect of TTX treatment on mean firing rate in MNs differentiated for 6 weeks n = 7 independent experiments. All data shown as mean ± SEM. ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001, ∗∗∗∗p < 0.0001. Two-way ANOVA. See also Figure S7.