Figure 7.

TDP-43 variants lead to decreased synapsin I protein levels but not SYN1 transcript levels

(A–D) Immunoblot (A) and quantification of protein levels of PSD95 (B), synapsin I (C), and synaptophysin (D) in MNPCs and MNs harvested after 2, 4, and 6 weeks of differentiation. βIII-tubulin was used as loading control.

(E–G) Longitudinal quantification of relative transcript levels of DLG4 (encoding PSD95) (E), SYN1 (F), and SYP (G) using qPCR. All data shown as mean ± SEM. ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001, ∗∗∗∗p < 0.0001. Two-way ANOVA. n = 3 independent experiments.