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. 2023 Nov 22;57(3):e13562. doi: 10.1111/cpr.13562

FIGURE 1.

FIGURE 1

DKK expression in mouse alopecia areata (AA). (A) The expression of DKK1 was significantly elevated in the skin tissue of AA mice (arrowheads, indicated by green). (A and B) Control mice skin tissue and (C and D) AA mice skin tissue. Scale bars indicate 10 μm. (B) The mRNA expression of DKK1 was assessed through in situ hybridization, and it appeared as pink (DKK1 mRNA, arrowheads) staining between adipocytes in the skin of AA mice. Haematoxylin (purple) was counterstained. (C) The mRNA expression level of DKK1 is high in AA mice back skin tissue. (D) DKK1 protein is specifically expressed in ASCs, which are also stained positive for the ASC marker PDGFRA (A–D) and CD34 (E–H) in AA mice skin tissue. Scale bars indicate 10 μm. (E) The mRNA expression level of DKK1 was evaluated in human DPCs and ASCs and significantly higher in ASCs. *p < 0.05, **p < 0.01. Three independent experiments were conducted for all data points. Error bars indicate the S.E.M.