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. 2005 May 6;33(8):2595–2602. doi: 10.1093/nar/gki546

Figure 2.

Figure 2

(A) Nucleotide sequences of selected clones of randomized tRNA after sixth and seventh in vitro selection cycles against antiterminator model RNA AM1A. Group I corresponds to the kissing tRNA sequences, while Group II corresponds to the functionally relevant tRNA sequences. (B) Nucleotide sequences of selected clones of randomized tRNA after the sixth and seventh in vitro selection cycles against antiterminator model RNA AM1A(C11U). For both (A) and (B), the parentheses next to the sequence number indicate the number of identical sequences. Sequences from randomized regions are shown in capital. Dots indicate the sequences identical to the wild-type B.subtilis tRNATyr(A73U). Lines indicate base deletion. Tertiary base pairing interactions are represented by color coding as shown for the wild-type B.subtilis tRNATyr(A73U) sequence. (See Materials and Methods for evaluation criteria and details regarding selection). Base pair color coding is as follows: 8:14, green; 15:48, red; 18:55, light blue; 19:56, dark blue; 54:58, pink.