Table 1.
Kd (μM) of representative selected tRNAs and mutants for binding antiterminator model RNAs
| tRNA | AM1Aa | AM1A(C11U)a |
|---|---|---|
| B.subtilis tRNATyr(A73U) | 63b | 200b |
| AM1A Group I (kissing) | ||
| G7–U12 | 0.3 | – |
| G7–U12(ACCA)c | 0.5 | – |
| G7–M12 | 0.3 | – |
| G7–M12(mTL)d | 134 ± 30 | – |
| G7–M12(mTL)(AGGU)c,d | n.d. | – |
| G7–M14 | 0.5 | – |
| G7–M14(ACCA)c | 0.5 | – |
| AM1A Group II (functionally relevant) | ||
| G7–M2 | 36 ± 4 | – |
| G7–M2(ACCA)c | n.d. | – |
| G6–M18 | 7 | – |
| G6–M18(ACCA)c | 11 ± 5 | – |
| AM1A(C11U) | ||
| G7–CU1 | 53 ± 22 | 5 |
| G7–CU1(ACCA)c | – | 7 |
| G7–CU5 | – | 26 ± 6 |
n.d., no binding detected; ‘—’, not applicable. All values ± 1.0 (unless otherwise noted) based on replicate measurements.
aValues (except as noted) determined by gel shift as described in Materials and Methods.
bValues from (8).
cSequence of acceptor end 5′–3′ introducing one (ACCA) or complete (AGGU) mismatch with antiterminator model RNAs.
dSequence in the T loop 5′-AAGGAAC-3′.