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. 2005 May 6;33(8):2620–2628. doi: 10.1093/nar/gki563

Table 2.

Extension past incorporated analogues

Template Analogue +2 dNTP Polymerase %Elongation
DNAG d5OMeBTP d5OMeBTP KF 3.1
DNAA d6OMeBTP d6OMeBTP KF 1.3
DNAG dOMeBTP d6OMeBTP KF 1.9
DNAA d6F3BTP d6F3BTP Pol α 0.9
DNAT d5OMeBTP TTP KF 2.5
DNAT d6OMeBTP TTP KF 2.5

Percent elongation indicates the amount of ‘primer +1’ DNA that was extended to ‘primer + 2’. Reactions contained 100 μM analogue dNTP, 100 μM TTP if indicated, and a 10-fold increase in polymerase relative to standard kinetic assays, with no correction in the resultant % extension.