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. 2023 Aug 2;9(2):e10584. doi: 10.1002/btm2.10584

FIGURE 1.

FIGURE 1

Design of mARGds HEK293T clones. (a) Schematic of the transposase integrating mARGds construct designs. From left to right, the XLone‐mARG1 cassette possesses the Blasticidin resistant gene and the Tet‐On 3G system downstream of the constitutive EF‐1α promoter, and the mARG GvpB gene and mCherry downstream of the Dox inducible TRE3GS promoter. The XLone‐mARG2 cassette possesses the Puromycin resistant gene and the Tet‐On 3G system downstream of the constitutive EF‐1α promoter, and the mARG GvpF, GvpG, GvpL, GvpS, GvpK, GvpJ, GvpU genes (separated by P2A) and GFP downstream of the Dox inducible TRE3GS promoter. Finally, PB‐UbC‐mARG3 cassette possesses the mARG GvpF, GvpG, GvpL, and GvpK genes downstream of the constitutive hUbC promoter and Geneticin resistant gene downstream of the constitutive PGK promoter. (b) Flow cytometry of mARGds HEK293T clonal lines against GFP (XLone‐mARG2) and mCherry (XLone‐mARG1).