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. 2023 Dec 25;40(4):337–344. doi: 10.5511/plantbiotechnology.23.0920a

Figure 2. Transactivation activity assay of LATE. (A) A schematic drawing of the plasmids. Effector plasmids containing the Gal4-DB-fused full-length coding sequence of LATE, LATEΔEAR, LATEΔEAR:VP16, LATE:VP16, or LATE:SRDX were cotransfected into protoplasts derived from Arabidopsis leaves with the reporter plasmid containing fLUC driven by 5×Gal4-binding sites and the reference plasmid containing rLUC. (B) Transactivation activities of LATE proteins. Transactivation activities were calculated by dividing fLUC activity by rLUC activity, and the activity of the control was set as 1. Bars indicate standard deviation of eight replicates. Bars with different letters a, b, and c are significantly different from each other (pairwise t-test with Holm correction, p<0.05).

Figure 2. Transactivation activity assay of LATE. (A) A schematic drawing of the plasmids. Effector plasmids containing the Gal4-DB-fused full-length coding sequence of LATE, LATEΔEAR, LATEΔEAR:VP16, LATE:VP16, or LATE:SRDX were cotransfected into protoplasts derived from Arabidopsis leaves with the reporter plasmid containing fLUC driven by 5×Gal4-binding sites and the reference plasmid containing rLUC. (B) Transactivation activities of LATE proteins. Transactivation activities were calculated by dividing fLUC activity by rLUC activity, and the activity of the control was set as 1. Bars indicate standard deviation of eight replicates. Bars with different letters a, b, and c are significantly different from each other (pairwise t-test with Holm correction, p<0.05).