The comparation of the MO results in healthy young and older participant groups. (a, b) MO imaging results of young and older participants. The MO imaging of phase 1 (a) and phase 3 (b) were both brighter in the younger healthy participant. (c) The statistical plot of mean MO results in young and older healthy participant groups. MO for no pulse trigger in the entrance of the foramen of Monro was higher in the young group. MO for phase 1 in the fourth ventricle, lateral ventricle, sylvian fissure, and anterior horn of the lateral ventricles were higher for young participants compared to the older group. MO for phase 3 in the lateral ventricle, entrance from the foramen of Monro, sylvian fissure, anterior horn of the lateral ventricle and area around superior sagittal sinus (SSS) was higher for young participants compared to the older group.