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. 2005 Apr 6;6:17. doi: 10.1186/1471-2121-6-17

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Copyright © 2005 Riess et al; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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APC is present in EB1-ΔN2-GFP aggresomes. Panels A-C. Cells expressing EB1-ΔN2-GFP were fixed and co-immunostained for GFP (green, panel A) and APC (red, panel B). A merged image is shown in panel C. APC is seen in aggresomes in transfected cells and at peripheral sites in adjacent untransfected cells. Bar = 10 μm. Panels D-F. A single 0.365 μm confocal section through a cell expressing EB1-ΔN2-GFP. APC immunoreactivity (panel E) in aggresomes is heterogeneous but only seen in association with GFP immunoreactivity (panels D and F). Bar = 5 μm. Panels G-I. Cells expressing EB1-ΔN2-GFP were fixed and co-immunostained for GFP (green, panel G) and EB3 (red, panel H). EB3 is seen at microtubule tips but is not present in EB1-ΔN2-GFP aggresomes. Bar = 20 μm. Images in panels A-C and G-I are projections of confocal image stacks.