FIG. 4.

NUT2 is an essential gene that exhibits synthetic lethality with NUT1. (A) NUT2 is an essential gene. Isogenic strains that are NUT2 (RT781) or nut2Δ (RT783) were maintained with a centromere plasmid containing URA3 and NUT2. Strains were streaked on synthetic complete medium (left) or on synthetic complete medium containing 5-FOA (right) and incubated for 3 days. (B) A temperature-sensitive allele of NUT2, nut2-ts70, is synthetically lethal with a deletion of NUT1. Isogenic strains derived from RT784 that are NUT1 NUT2 (RT784-6b), nut1Δ NUT2 (RT784-1d), NUT1 nut2-ts70 (RT784-1a), and nut1Δ nut2-ts70 (RT784-2c) were constructed bearing pRKT353, a centromere plasmid containing the URA3 and NUT1 genes. Strains were streaked on plates with (center) or without (left and right) 5-FOA and incubated at 25°C (left and center) or 37°C (right) for 3 days.