TABLE 1.
Yeast strains useda
| Strain | Genotype |
|---|---|
| W303ab | MATa ade2 his3 leu2 ura3-52 trp1 can1 ho |
| K1107 | HMLa MATa HMRa his3 leu2 ura3-52 trp1 can1 ho-lacZ46 ade− |
| RT228 | HMLa MATa HMRa swi4ΔHIS3 his3 leu2 ura3-52 trp1 can1 ho ade− |
| RT233c | MATa ho::uasΔURA3::ho-lacZ |
| RT241c | MATα SWI4 LEU2::pRKT188 |
| RT243 | MATa swi4ΔHIS3 NUT LEU2::pRKT188 |
| RT259 | MATa swi4ΔHIS3 nut9-1 LEU2::pRKT188 |
| RT267 | MATa swi4ΔHIS3 nut7-2 LEU2::pRKT188 |
| RT269 | MATa swi4ΔHIS3 nut3-1 LEU2::pRKT188 |
| RT271 | MATa swi4ΔHIS3 nut1-1 nut2-1 LEU2::pRKT188 |
| RT300c | MATa swi4ΔHIS3 sin4ΔLEU2 LEU2::pRKT188 |
| RT335c | MATa sin4ΔLEU2 ho::uasΔURA3::ho-lacZ |
| RT362c | MATα swi4ΔTRP1 nut3-1 LEU2::pRKT188 |
| RT364c | MATα swi4ΔHIS3 nut1-1 nut2-1 LEU2::pRKT188 |
| RT379c | MATa swi4ΔHIS3 nut8-1 LEU2::pRKT188 |
| RT381c | MATa swi4ΔHIS3 nut6-2 LEU2::pRKT188 |
| RT387c | MATα swi4ΔTRP1 nut7-2 LEU2::pRKT188 |
| RT420c | MATα swi4ΔHIS3 nut1-1 NUT2 LEU2::pRKT188 |
| RT421c | MATα swi4ΔHIS3 NUT1 nut2-1 LEU2::pRKT188 |
| RT564 | MATa SWI4 NUT1 NUT2 LEU2::pRKT188 |
| RT575b | MATa/α |
| RT576b | MATa/α nut2ΔhisG::URA::hisG/NUT2 |
| RT609 | MATα swi4ΔHIS3 nut1ΔTRP1 nut21-609 LEU2::pRKT188 |
| RT688 | MATa SWI4 LEU2::pRKT229 |
| RT689 | MATa swi4ΔHIS3 LEU2::pRKT229 |
| RT692 | MATa SWI4 LEU2::pRKT208 |
| RT693 | MATa swi4ΔHIS3 LEU2::pRKT208 |
| RT718 | MATa swi4ΔHIS3 nut1ΔTRP1 nut2-1::URA3 LEU2::pRKT229 |
| RT720 | MATα SWI4 nut1ΔTRP1 nut2-1::URA3 LEU2::pRKT229 |
| RT748 | MATa swi4ΔHIS3 NUT1 nut2-ts70 LEU2::pRKT229 |
| RT781b | MATa NUT2 pRKT517 |
| RT783b | MATa nut2ΔhisG pRKT517 |
| RT784 | MATa/α nut1ΔTRP1/NUT1 nut2-ts70/NUT2 LEU2::pRKT229/LEU2::pRKT229 pRKT353 |
| RT784-1a | MATα NUT1 nut2-ts70 LEU2::pRKT229 pRKT353 |
| RT784-1d | MATα nut1ΔTRP1 NUT2 LEU2::pRKT229 pRKT353 |
| RT784-2c | MATα nut1ΔTRP1 nut2-ts70 LEU2::pRKT229 pRKT353 |
| RT784-6b | MATα NUT1 NUT2 LEU2::pRKT229 pRKT353 |
| RT817 | MATa swi4ΔHIS3 srb10ΔURA3 LEU2::pRKT229 |
| RT821 | MATa swi4ΔHIS3 srb11ΔURA3 LEU2::pRKT229 |
| RT849c | MATa srb10ΔURA3 ho::uasΔURA3::ho-lacZ |
| RT850c | MATa nut1ΔTRP1 nut2-1::URA3 ho::uasΔURA3::ho-lacZ |
| RT854 | MATa swi4ΔHIS3 srb8ΔURA3 LEU2::pRKT229 |
| RT890 | MATa urs2LΔho |
| RT930 | MATa swi4ΔHIS3 srb9ΔURA3 LEU2::pRKT229 |
| RT952 | MATa swi4ΔHIS3 NUT urs2LΔho |
| RT953 | MATa swi4ΔHIS3 nut1ΔTRP1 nut2-1::URA3 urs2LΔho |
| RT954c | MATa swi4ΔHIS3 nut1ΔTRP1 nut2-1::URA3 urs2LΔho-lacZ |
| RT973c | MATa SWI4 NUT urs2LΔho-lacZ |
| RT976c | MATa swi4ΔHIS3 urs2LΔho-lacZ |
| RT1118 | MATa swi4ΔHIS3 srb10Δ urs2LΔho |
| RT1119c | MATa swi4ΔHIS3 sin4ΔLEU2 urs2LΔho-lacZ |
| RT1120c | MATa swi4ΔHIS3 sin4ΔLEU2 urs2LΔho |
a
All strains are isogenic to RT228 except as noted otherwise. urs2LΔ is referred to in the text as URS1-URS2R.
b
Isogenic to W303.
c
Derived from K1107 and W303.