TABLE 2.
Plasmids used
| Name | Description |
|---|---|
| pRKT186 | pBR322.ho::uasΔURA3::ho-lacZ |
| pRKT208 | pRS305.UASGAL-lacZ |
| pRKT229, pRKT188 | pRS305.UASGAL-URS2R-lacZ |
| pRKT353 | YCp50.NUT1 |
| pRKT354 | YCp50.NUT1ΔSalI-SphI |
| pRKT355 | YCp50.NUT1ΔSalI-XbaI |
| pRKT356 | pRS306.2.3kb HindIII adjacent to NUT1 |
| pRKT363 | pGEM11.NUT1 |
| pRKT365 | pGEM11.nut1ΔTRP1 |
| pRKT404 | YCp50.NUT2 |
| pRKT417 | pRS306.NUT2 |
| pRKT427 | pRS306.SWI4 |
| pRKT432 | pBluescript.nut2ΔhisG.URA3.hisG |
| pRKT439 | YCp50.NUT7/SRB10 |
| pRKT447 | YCp50.NUT2(EcoRIΔ) |
| pRKT448 | YCp50.NUT2(XbaI-SphIΔ) |
| pRKT449 | YCp50.NUT2(AflIIΔ) |
| pRKT450 | YCp50.NUT2(XbaI-AflII) |
| pRKT515 | pRS306.nut2-1 |
| pRKT517 | pRS316.NUT2 |
| pRKT519 | YCp50.nut2-1 |
| pRKT535 | pRS306.URA3.NUT2-HA |
| pRKT542 | pRS306.URA3.HA-NUT1 |
| pRKT559 | pRS306.nut2-ts70 |
| pRKT562 | YCp50.NUT21 |
| pRKT564 | YCp50.NUT21(ΔSalI-NotI) |
| pRKT566 | pRS306.NUT21(SpeI-NotI) |
| pRKT619 | pRS306.urs2LΔho |