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. 2024 Mar 1;43:66. doi: 10.1186/s13046-024-02993-1

Fig. 3.

Fig. 3

CRISPR/Cas9-mediated editing of CAR T-cell. Production of CAR T cell starting from patient for autologous infusion (black line) including apheresis from peripheral blood and cryo-conservation; thawing is followed by stimulation and lentiviral transduction for generation of CAR T-cells. The expansion is achieved by bioreactors and the requested number of CAR T-cells is ready for patient infusion. The process starting from healthy donor (red line) for allogenic CAR T cell production follows the same procedure until CAR transduction. CRISPR/Cas9 editing of HLA and TCR before CAR T-cells expansion generates universal CAR T-cells that can be infused into the patient