Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2018 Mar 15;56(7):857–867. doi: 10.1093/mmy/myx135

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2018. Published by Oxford University Press on behalf of The International Society for Human and Animal Mycology.

This article is published and distributed under the terms of the Oxford University Press, Standard Journals Publication Model (https://academic.oup.com/journals/pages/open_access/funder_policies/chorus/standard_publication_model)

PMC Copyright notice

Figure 3. — Two C. gattii clinical isolates are resistant to flucytosine but retain the ability to assimilate cytosine. (A) Growth of 5FC resistant isolates (B9322 and JS5), 5FC susceptible isolate (JS91) and S. cerevisiae control strains (WT and fcy1Δ) on minimal media (SD) containing 2.5 μg/ml 5FC. Serial dilutions of cells (10⁵ cells/5 μl to 10¹ cells/5 μl) were spotted on SD agar supplemented with 2.5 μg/ml 5FC. (B) B9322 and JS5 retain the ability to assimilate cytosine as a sole nitrogen source. Serial dilutions of cells (10⁵ cells/5μl to 10¹ cells/5μl) were spotted on SD agar supplemented with 1–4 mM of cytosine as the sole nitrogen source. SD denotes synthetic dextrose minimal media. SD (-N) denotes synthetic dextrose media lacking a nitrogen source (NH₄SO₄).