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. 2018 Mar 15;56(7):857–867. doi: 10.1093/mmy/myx135

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© The Author(s) 2018. Published by Oxford University Press on behalf of The International Society for Human and Animal Mycology.

This article is published and distributed under the terms of the Oxford University Press, Standard Journals Publication Model (https://academic.oup.com/journals/pages/open_access/funder_policies/chorus/standard_publication_model)

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Figure 5. — Heterologous expression of C. gattii FCY1 wildtype (CgFCY1^wt) and FCY1 mutant (CgFCY1^mut) proteins under the control of the weak ADH promoter in the S. cerevisiae fcy1Δ genetic background (ScΔ). (A) Growth of S. cerevisiae wildtype, S. cerevisiae fcy1Δ, and heterologously expressed strains on minimal media (SD) in the presence of 2.5–10 μg/ml flucytosine (5FC). (B) Growth of S. cerevisiae strains on minimal media containing 1–4 mM cytosine as a sole nitrogen source. SD(-N) denotes synthetic dextrose agar lacking a nitrogen source.