Figure 6.
Heterologous expression of C. gattii FCY1 wildtype (CgFCY1wt) and FCY1 mutant (CgFCY1mut) proteins under the control of the weak ADH promoter in the S. cerevisiae fcy1Δ genetic background (ScΔ). Growth of S. cerevisiae wild-type, S. cerevisiae fcy1Δ, and heterologously expressed strains on minimal media (SD) in the presence of 0.0025–0.16 μg/ml flucytosine (5FC).