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. 2018 Mar 15;56(7):857–867. doi: 10.1093/mmy/myx135

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© The Author(s) 2018. Published by Oxford University Press on behalf of The International Society for Human and Animal Mycology.

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Figure 7. — Expression of C. neoformans and C. gattii FCY1 wildtype and FCY1 mutant proteins under the control of the actin promoter in the KN99 fcy1Δ genetic background (KN99Δ). Growth of KN99 wildtype, KN99 fcy1Δ, and heterologously expressed strains on minimal media (SD) in the presence of 5 μg/ml flucytosine (5FC) or 4 mM cytosine as a sole nitrogen source. CgFCY1^wt/CgFCY1^mut and CnFCY1^wt/CnFCY1^mut denote FCY1 wild-type and mutant proteins from C. gattii and C. neoformans, respectively. SD(-N) denotes synthetic dextrose agar lacking a nitrogen source.