Table 2.

Products used for characterization.

Product	Product number	Manufacturer	Use	Parameters
Anti-GFAP antibody	ab7260	Abcam	ICC	ICC: 1:1000
ACSA-2 Antibody, anti-mouse, PE, REAfinity™	130–116-141	Miltenyi Biotec	ICC and Flow Cytometry	Flow: 1:25, ICC: 1:50
GLAST (ACSA-1) Antibody, anti-human/mouse/rat, PE	130–118-344	Miltenyi Biotec	ICC and Flow Cytometry	Flow: 1:25, ICC: 1:50
GFAP Antibody, anti-human/mouse/rat, REAfinity™ (APC)	130–123-846	Miltenyi Biotec	Flow Cytometry	Flow: 1:25
recombinant IgG1 monoclonal antibodies, or with recombinant human IgG1 PE+ and APC+ conjugated isotype control antibodies	130–113-438 &130–113-434	Miltenyi Biotec	Flow Cytometry	Flow: 1:25
EAAT2 Polyclonal Antibody	BS-1751R	ThermoFisher	Flow Cytometry	Flow: 1:50
Anti-EAAT2 antibody	ab41621	Abcam	ICC	ICC: 1:500
TMEM119 Rabbit anti-mouse IgG polyclonal antibody	PA5-119902	Invitrogen	ICC and Flow Cytometry	Flow: 1:500, ICC: 1:500
IBA-1 recombinant rabbit anti-mouse monoclonal antibody [EPR16588]	ab178846	Abcam	ICC and Flow Cytometry	Flow: 1:160, ICC: 1:500
Donkey anti-Rabbit IgG (H + L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor™ Plus 488	A32790	Invitrogen	ICC and Flow Cytometry	Flow: 1:500, ICC: 1:500
Goat anti-Rabbit IgG (H + L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor™ Plus 555	A32732	Invitrogen	ICC and Flow Cytometry	Flow: 1:1000, ICC: 1:1000
Goat anti-Rabbit IgG (H + L) Cross-Adsorbed Secondary Antibody, Alexa Fluor™ 568	A-11011	Invitrogen	Flow Cytometry	Flow: 1:500
Rabbit IgG (H + L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor™ Plus 647	A32733	Invitrogen	ICC	ICC: 1:1000
Ibidi USA Supplier Diversity Partner Ibidi Mounting Medium With DAPI, 15 mL	NC1943852	Fisher	ICC	7 drops/35 mm plate, 4 drops per 24-well well
Image-IT™ Fixative Solutions	I28800	Invitrogen	Cell Fixation	Flow/ICC
Triton™ X-100	T8787-100 mL	Sigma-Aldrich	Cell Permeabilization	Flow/ICC
Phosphate Buffer Saline (PBS)	10,010,049	Gibco	General	General