Table 3.

Products used for functional assessments.

Product	Product number	Manufacturer	Use	Parameters
bEnd.3	CRL-2299	ATCC	TEER	6×104 cells/cm2
C8-D1A	CRL-2541	ATCC	TEER	Astrocyte Conditioned Media
Collagen from calf skin	C8919-20ML	Sigma/Millipore	TEER	1:4 in nanopure water
DMEM high glucose, pyruvate	11,995–073	ThermoScientific	TEER	500 mL
10% fetal bovine serum	10,082,139	Gibco	TEER	50 mL
L-glutamine	25,030,081	ThermoScientific	TEER	2 mM
Transwell-sterile	3,470	Costar Corning Inc.	TEER	24 well plates
xCT antibody-BSA Free	NB300-317	Novus	Immunoblotting	1:1000
α-tubulin antibody	3,873 s	Cell Signaling	Immunoblotting	1:5000
LI-COR IRDye 800CW Donkey anti-Rabbit	NC9523609	Fisher/LI-COR	Immunoblotting	1:10000
LI-COR IRDye 800CW Donkey anti-Mouse	NC0250903	Fisher/LI-COR	Immunoblotting	1:10000
MitoTracker™ Red CMXRo	M7512	Invitrogen	mitochondria visualization	final concentration of 250 nM in media
iScript cDNA Synthesis Kit	1,725,038	BioRad	qPCR	see manufacturer’s protocol
SsoAdvanced Universal SYBR Green Supermix	1,725,272	BioRad	qPCR	see manufacturer’s protocol
Phosphate Buffer Saline (PBS)	10,010,049	Gibco	General	General
ADRB2 forward primer	N/A	IDT	qPCR	5’-GTACCGTGCCACCCACAAGA-3’
ADRB2 reverse primer	N/A	IDT	qPCR	5’-CCCGGGAATAGACAAAGACCATC-3’
β-actin forward primer	N/A	IDT	qPCR	5′-GGCCAGGTCATCACTATTG-3′
β-actin reverse primer	N/A	IDT	qPCR	5′-GAGGTCTTTACGGATGTCAAC-3′