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. 2024 Jan 23;9(2):e173863. doi: 10.1172/jci.insight.173863

Figure 7. ABT-263 dose-dependently reduced endothelial cell viability.

Figure 7

(A) HAEC obtained from ATCC were treated with Dox for 1 day+ 7 days of recovery; they were then treated with different concentrations (0.1 μM, 1 μM, and 10 μM) of ABT-263, and representative photographs at the end of the treatment were taken with Leica of 10× zoom. Scale bar: 1 μm. (B) At the end of the treatment, we aspirated the media and performed the SAβG assay as described in Methods, and images were taken in 10× zoom of Leica microscope. Scale bar: 1 μm. (C and D) Percentage of SMC cell viability was measured with trypan blue assay with and without Dox and ABT-263. (E and F) Percentage of HAEC senescence measured from B. The 1-way ANOVA method was used for statistical analyses in C–F. Each well of the plate indicated as individual dots. Data are shown as mean ± SEM. The P values are indicated on the respective graphs. All the cell culture experiments in this figure were repeated 3 times.