FIG. 3.

The B box is the primary site of importin/karyopherin-α recognition. Each of the wild type and mutant HMG DNA binding domains depicted at the bottom was generated by in vitro translation in reticulocyte lysates supplemented with [³⁵S]methionine. Protein equivalent amounts of each translation product were incubated with either GST-pendulin or GST alone in the GST pull-down assay. A portion (10%) of the amount of each translation product added to the binding assay is shown in the bottom panels.