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In vitro analysis of the pendulin deletion constructs. Proteins encoded by N-terminal (A) and C-terminal (B) deletion constructs of pendulin were generated by translation in vitro in reticulocyte lysates supplemented with [35S]methionine. Translation products were tested for interaction with GST–LEF-1, GST–TCF-1, and GST alone in the GST pull-down assay. Protein equivalent amounts of each translation product were added to the binding reactions as shown in the bottom panel.
