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LEF-1 but not TCF-1 can enter the nucleus in digitonin-permeabilized HeLa cells. Recombinant GST-GFP fusions of the LEF-1 and TCF-1 HMG DNA binding domains were purified and added to digitonin-permeabilized HeLa cells in the presence of a HeLa S100 cytosol extract with or without ATP and an energy regeneration system. Purified GST-pendulin protein (0.8 μg/180 nM) was added to the transport assay shown in the bottom right panel.
