Extended Data Fig. 3 |. Multiple sequence alignment of FezB with characterized P_1B-ATPases.

Conserved functional motifs in the actuator domain and the ATP-binding domain are indicated with blue and purple stars, respectively. The CxxC and histidine-rich metal binding sites in the cytoplasmic N-terminal domain of ZntA, CopA, and CopB are boxed. Transmembrane regions, predicted using TOPCONS 1.0⁶⁵, are underlined for each sequence. Putative metal-binding sites in the transmembrane domains are indicated with black stars.