Fig. 2.

Alcohol’ effects on tumorigenesis and aggressiveness of breast cancer. A: Adolescent and adult FVB MMTV-Wnt1 mice received chronic alcohol exposure through liquid diet. When tumor size reached maximal diameter of 20 mm, the mice were euthanized, and the number of mice bearing mammary tumor and lung metastasis were determined. For adolescent alcohol exposure, n = 20 for control group and n= 19 for alcohol exposure group. For adult alcohol exposure, n = 15 for both control and alcohol-exposed group. *p< 0.05 as determined by the Fisher exact test. B. Adolescent FVB MMTV-Wnt1 mice received either acute alcohol exposure through IP injection or chronic alcohol exposure through liquid diet as described above. When tumors in mice reached 20 mm maximal diameter they were euthanized, number of mice bearing multiple tumors (more than 2 tumors/mouse) and lung metastasis were determined. For chronic exposure, n = 11 for control group and n = 14 for alcohol group. For acute exposure, n = 8 for both control and alcohol exposure group. * significant difference from controls, # significant difference from chronic exposure group, p< 0.05 as determined by the Fisher exact test. C. Representative images of lung metastasis. Adolescent FVB MMTV-Wnt1 mice received chronic exposure through liquid diet as described above. Mice were euthanized and lung tissues were obtained for Hematoxylin and Eosin (H&E) staining. Arrows indicate the metastases.