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. Author manuscript; available in PMC: 2024 Mar 1.
Published in final edited form as: Alcohol Clin Exp Res (Hoboken). 2022 Dec 10;47(2):251–262. doi: 10.1111/acer.14986

Fig. 3.

Fig. 3.

Alcohol’s effects on cancer stem cells (CSCs) in mammary tumors. Adolescent and adult MMTV-Wnt1 mice received chronic alcohol exposure through liquid diet or acute alcohol exposure through gavage as described above. When tumors in mice reached 20 mm maximal diameter, they were sacrificed, and mammary tumor cells were isolated for assaying CSC population. CSCs were determined by activity of aldehyde dehydrogenase (ALDH) using ALDEFLUOR kit. A. CSC population was determined following chronic alcohol exposure. n = 6 for each group. * significant difference from controls, p < 0.05. B. CSC population was determined following acute alcohol exposure in adolescent mice. n = 7 for each group. * significant difference from controls, p < 0.05.